Practitioner Fellowship

Analysis of prion protein conformation using circular dichroism spectroscopy

LJ Ellett, VA Johanssen

2017-01-01

According to the protein-only hypothesis of prion propagation, the pathogenesis of prion disease is due to the misfolding of cellu..

C-terminal peptides modelling constitutive PrPC processing demonstrate ameliorated toxicity predisposition consequent to α-cleavage

VA Johanssen, T Johanssen, CL Masters, AF Hill, KJ Barnham, SJ Collins

2014-04-01

Misfolding of PrP^C (cellular prion protein) to β-strand-rich conformations constitutes a key event in prion disease pat..

Prion subcellular fractionation reveals infectivity spectrum, with a high titre-low PrP res level disparity

V Lewis, CL Haigh, CL Masters, AF Hill, VA Lawson, SJ Collins

2012-04-27

Background: Prion disease transmission and pathogenesis are linked to misfolded, typically protease resistant (PrP ^res)..

Cellular prion protein expression is not regulated by the Alzheimer's amyloid precursor protein intracellular domain

V Lewis, IJ Whitehouse, H Baybutt, JC Manson, SJ Collins, NM Hooper

2012-02-21

There is increasing evidence of molecular and cellular links between Alzheimer's disease (AD) and prion diseases. The cellular pri..

Acute exposure to prion infection induces transient oxidative stress progressing to be cumulatively deleterious with chronic propagation in vitro

CL Haigh, AR McGlade, V Lewis, CL Masters, VA Lawson, SJ Collins

2011-08-01

Neuronal loss is a pathological feature of prion diseases for which increased reactive oxygen species (ROS) and consequent oxidati..

Optical imaging detects apoptosis in the brain and peripheral organs of prion-infected mice

SC Drew, CL Haigh, HMJ Klemm, CL Masters, SJ Collins, KJ Barnham, VA Lawson

2011-02-01

Activation of the caspase family of cysteine proteases is proposed tobe an important cell death mechanism in transmissible spongif..

Near-infrared fluorescence imaging of apoptotic neuronal cell death in a live animal model of prion disease

VA Lawson, CL Haigh, B Roberts, VB Kenche, HMJ Klemm, CL Masters, SJ Collins, KJ Barnham, SC Drew

2010-11-17

Apoptotic cell death via activation of the caspase family of cysteine proteases is a common feature of many neurodegenerative dise..

Glycosaminoglycan sulphation affects the seeded misfolding of a mutant prion protein

VA Lawson, B Lumicisi, J Welton, D Machalek, K Gouramanis, HM Klemm, JD Stewart, CL Masters, DE Hoke, SJ Collins, AF Hill

2010-10-20

Background: The accumulation of protease resistant conformers of the prion protein (PrP^res) is a key pathological featu..

Manganese chelation therapy extends survival in a mouse model of M1000 prion disease

MW Brazier, I Volitakis, M Kvasnicka, AR White, JR Underwood, JE Green, S Han, AF Hill, CL Masters, SJ Collins

2010-07-01

Previous in vitro and in vivo investigations have suggested manganese (Mn²⁺⁾ may play a role in pathogenesis through fa..

Copper, endoproteolytic processing of the prion protein and cell signalling

CL Haigh, SY Marom, SJ Collins

2010-06-01

Recently, understanding of many molecular interactions has progressed appreciably and cellular events once thought to be by-produc..

No evidence for prion protein gene locus multiplication in Creutzfeldt-Jakob disease

SJ Collins, M Schuur, A Boyd, V Lewis, GM Klug, A McGlade, A van Oosterhout, G Breedveld, BA Oostra, C Masters, CM Van Duijn

2010-03-12

Precedent of causative multiplication of key gene loci exists in familial forms of both Alzheimer's and Parkinson's diseases. Gene..

Increased proportions of c1 truncated prion protein protect against cellular m1000 prion infection

V Lewis, AF Hill, CL Haigh, GM Klug, CL Masters, VA Lawson, SJ Collins

2009-10-01

Prion disease pathogenesis is linked to the cell-associated propagation of misfolded protease-resistant conformers (PrP) of the no..

PrP C -related signal transduction is influenced by copper, membrane integrity and the alpha cleavage site

CL Haigh, VA Lewis, LJ Vella, CL Masters, AF Hill, VA Lawson, SJ Collins

2009-09-01

The copper-binding, membrane-anchored, cellular prion protein (PrP C) has two constitutive cleavage sites producing distinct N- an..

A manganese-superoxide dismutase/catalase mimetic extends survival in a mouse model of human prion disease

MW Brazier, SR Doctrow, CL Masters, SJ Collins

2008-07-15

Animal models, and human postmortem studies, of prion disease have demonstrated the presence of heightened oxidative stress in the..

Mouse-adapted sporadic human Creutzfeldt-Jakob disease prions propagate in cell culture

VA Lawson, LJ Vella, JD Stewart, RA Sharples, H Klemm, DM Machalek, CL Masters, R Cappai, SJ Collins, AF Hill

2008-06-30

Cell based models used for the study of prion diseases have traditionally employed mouse-adapted strains of sheep scrapie prions. ..